Contact between European bison and cattle from the cattle breeders' perspective, in the light of the risk of pathogen transmission.

Pathogens transmitted between wildlife and domestic animals can pose a threat to endangered species, undermine conservation efforts in wildlife, and affect productivity and parasite control in domestic animals. There are several examples of pathogen transmission between European bison and other animals. The present study surveyed breeders from the vicinity of four large wisent populations in eastern Poland about observed contacts between wisent and cattle. Such contacts were noted by 37% of breeders, indicating a significant risk of contact between European bison and cattle in the study areas, even in the areas where the European bison live mainly in a forest complex, i.e., in the Borecka Forest. A higher potential risk of contacts between European bison and cattle was noted in the Bialowieska Forest and the Bieszczady Mountains than in the Borecka and Knyszynska Forests. In the Bialowieska Forest, the risk of viral pathogen transmission resulting from contacts is higher (more direct contacts), and in the case of the Bieszczady Mountains, the probability of parasitic diseases is higher. The chance of contacts between European bison and cattle depended on the distance of cattle pastures from human settlements. Moreover, such contact was possible throughout the year, not only in spring and fall. It appears possible to minimize the risk of contacts between wisent and cattle by changing management practices for both species, such as keeping grazing areas as close as possible to settlements, and reducing the time cattle graze on pastures. However, the risk of contact is much greater if European bison populations are large and are dispersed beyond forest complexes.

Demand for outdoor recreation in a time of a pandemic / La demande pour des espaces de nature en situation de pandémie

INTRODUCTION: The extent of the health benefits of contact with nature came to the fore during the health crisis. However, studies do not fully appreciate the effect of the type of natural environment to which individuals are exposed. The studies often use a very vague category of "green space" for the purpose. PURPOSE OF RESEARCH: We apply social sciences analytical concepts to analyze demands for recreational uses of both forests and ocean beaches in a time of sanitary crisis. Our studies use data from two regional surveys applied to a representative sample of the Aquitaine population. RESULTS: We underline the social inequalities with regard to access to forest and ocean beaches, even though outdoor recreation is free of charge most of the time. We also identify salient differences between uses, motivations, and risk perceptions in both of the natural settings. We discuss how such discrepancies are inherited from previously constructed social representations. CONCLUSIONS: We believe that public health studies could benefit greatly from the achievements of several decades of research in the field of outdoor studies.

Introduction: La crise sanitaire que nous traversons est une occasion de mettre en lumière l'étendue des bienfaits sur la santé que procure le contact avec la nature. Dans ce contexte, néanmoins, les études négligent encore très souvent l'impact du type de milieu naturel que les individus fréquentent, retenant pour l'occasion une catégorie très générale d'« espaces verts ¼. But de l'étude: À travers deux enquêtes quantitatives portant sur la fréquentation des forêts et des plages océanes en Aquitaine, nous analysons, à l'aide d'outils empruntés aux sciences sociales, la façon dont chacun des deux espaces a répondu à des demandes spécifiques durant la première année de l'épidémie de COVID-19. Résultats: Nous montrons que les espaces se distinguent du point de vue des publics qui les fréquentent, des usages qu'ils permettent, mais aussi des attentes et des perceptions dont ils sont l'objet en matière de risques de contamination. Plus accessibles que d'autres formes de loisirs, les activités de plein air n'en demeurent pas moins un cas d'inégalité environnementale. Chez nos enquêtés, la forêt bénéficie d'une image plus positive que le littoral en termes de bien-être et de bénéfices pour la santé, y compris au niveau des risques de contamination par le SARS-CoV-2. Ces résultats renforcent l'idée qu'il existe effectivement des spécificités à la « santé verte ¼ (Green Health), d'un côté, et à la « santé bleue ¼ (Blue Health), de l'autre. Savoir d'où proviennent ces différences de représentations demeure une question ouverte, en émettant toutefois l'hypothèse que les croyances sont, au moins en partie, héritées de références constituées antérieurement à la pandémie, notamment sur la nature et le poids des fonctions environnementales. Conclusions: Les rapports que les individus entretiennent avec la nature résultent d'entrelacs complexes de facteurs environnementaux et sociaux. Ces relations influent sur les bénéfices qu'ils retirent de la fréquentation des espaces naturels. Dans ces conditions, les travaux de santé publique pourraient tirer profit des acquis obtenus depuis maintenant plusieurs décennies par les recherches en sciences sociales sur les activités de plein air.

How Do Repeated Viewings in Forest Landscapes Influence Young People's Visual Behaviors and Cognitive Evaluations?

BACKGROUND: With the spread of the COVID-19 epidemic, it has gradually become normal to periodically visit and enjoy forest landscape resources in the suburbs of cities. For designers and managers of forest landscapes, exploring change in the visual behaviors and cognitive evaluations of people who repeatedly view forest landscapes and the characteristics of this change will aid the design and sustainable utilization of forest landscape resources in the suburbs of cities. PURPOSE: From the perspective of users' preferences for forest landscape space, this study explored the changes in visual behavior characteristics and psychological preference characteristics for individuals who repeatedly view forest landscapes and their drivers under different preferences. METHODS: This study collected data from 52 graduate and undergraduate students. We used a difference test to compare the differences in the visual behavior coincidence degree and the changes in psychological evaluations; a descriptive statistical analysis to explore young peoples' likes and dislikes of landscape elements; and Spearman correlation analysis to explore the correlation between the psychological evaluations and visual behaviors. MAIN RESULTS: 1. At the second viewing, the participants' regression behavior tended to decrease for various spaces, and they were more inclined to view areas that they had not viewed before. In addition, at the second viewing, the degree of fixation behavior coincidence was generally low, and there were obvious differences across spaces; 2. The participants' feature evaluations and comprehensive evaluations for landscapes did not change significantly with their increased familiarity with the spaces; 3. There was a significant positive correlation between the participants' psychological evaluations of landscape stimuli and the degree of fixation coincidence when viewing the spaces, among which the rate of distant clarity and the degree of fixation behavior coincidence were significantly and positively correlated. Meanwhile, at the second viewing, the number of favorite elements in the lookout space, which belongs to high-preference spaces, noticeably increased.

Natural land cover positively correlates with COVID-19 health outcomes.

BACKGROUND: The coronavirus disease 2019 (COVID-19) poses special challenges for societies, as the disease causes millions of deaths. Although the direct prevention measures affect the prevalence and mortality the most, the other indirect factors, including natural environments and economics, could not be neglected. Evaluating the effect of natural land cover on COVID-19 health outcomes is an urgent and crucial public health topic. METHODS: Here, we examine the relationships between natural land cover and the prevalence and mortality of COVID-19 in the United States. To probe the effects of long-term living with natural land cover, we extract county-level land cover data from 2001 to 2019. Based on statistically spatial tests, we employ the Spatial Simultaneous Autoregressive (SAC) Model to estimate natural land cover's impact and monetary values on COVID-19 health outcomes. To examine the short-term effects of natural environments, we build a seasonal panel data set about the greenery index and COVID-19 health outcomes. The panel SAC model is used to detect the relationship between the greenery index and seasonal COVID-19 health outcomes. RESULTS: A 1% increase in open water or deciduous forest is associated with a 0.004-death and 0.163-conformed-case, or 0.006-death and 0.099-confirmed-case decrease in every 1,000 people. Converting them into monetary value, for the mortality, a 1% increase in open water, deciduous forest, or evergreen forest in a county is equivalent to a 212-, 313-, or 219-USD increase in household income in the long term. Moreover, for the prevalence, a 1% change in open water, deciduous forest, or mixed forest is worth a 382-, 230-, or 650-USD increase in household income. Furthermore, a rational development intensity is also critical to reduce the risk of the COVID-19 pandemic. More greenery in the short term is also linked to lower prevalence and mortality. CONCLUSIONS: Our study underscores the importance of incorporating natural land cover as a means of mitigating the risks and negative consequences of future pandemics like COVID-19 and promoting overall public health.

Analysis of the Relations Between Forestry Financial Supports and Forest Crimes.

Forest crimes are among the serious threats destroying forests. To prevent the forest crimes there are various solutions proposed, such as fortification of the laws, increasing the penalties, or increasing the public awareness. This article, however, suggests an alternative solution of preventing the forest crimes by investigating the relations between the individual financial supports provided to forest villagers and the levels of various forest crime types in Turkey. The study shows that, when the forest villagers are given financial supports, the levels of illegal logging, illegal transferring of forest products, illegal expansion of private lands into forests, illegal processing of trees, and illegal pasturage crimes decrease significantly. However, the financial supports do not affect the levels of illegal occupation of forestlands crime.

International modified Delphi study on malaria preventive behaviour: new themes, contexts and framework for future research on Plasmodium knowlesi malaria.

INTRODUCTION: Plasmodium knowlesi malaria is a non-human simian malaria that threatens Southeast Asian rural communities. Studies indicate that non-compliant bednet usage, travelling into the forest and working as farmers and rubber tappers put communities at risk for infection. Despite guidelines, malaria incidence increases yearly and has become a public health concern. In addition to research gaps addressing factors that influence malaria prevention behaviour in these communities, there are no specific guidelines to facilitate strategies against the threat of P. knowlesi malaria. METHOD: To examine potential factors that influence malaria-prevention behaviour in communities exposed to P. knowlesi malaria, 12 malaria experts participated in a modified Delphi study; every participant maintained their anonymity throughout the study. Three Delphi rounds were conducted via different online platforms between 15 November 2021 and 26 February 2022, and consensus was achieved when 70% of the participants agreed on a particular point with a 4-5 median. The results from the open-ended questions were then subjected to thematic analysis, and the dataset generated by this study was analysed using a deductive and inductive approach. RESULTS: After a systematic, iterative process, knowledge and belief, social support, cognitive and environmental factors, past experience as a malaria patient, and the affordability and feasibility of a given intervention were critical contributors to malaria-prevention behaviour. CONCLUSION: Future research on P. knowlesi malaria could adapt this study's findings for a more nuanced understanding of factors that influence malaria-prevention behaviour and improve P. knowlesi malaria programmes based on the expert consensus.

Increased appreciation of forests and their restorative effects during the COVID-19 pandemic.

Public expectations of forests as high-quality restorative environments that facilitate subjective well-being and stress relief along with numerous health benefits have been rising sharply during recent decades. In addition, the COVID-19 pandemic and its accompanying restrictive measures also transformed forests into some of the few places to spend time away from home. The presented study drew on the assumption that the pandemic situation and a rise in the number of forest visits would affect the experience, recognition, and appreciation of the well-being aspects related to spending time in forests. The study goal was to elucidate the potential effects of the COVID-19 pandemic on the relationships between forest visits, well-being and stress relief, emotions, perception of nature and forest value and importance, pro-environmental behavior, and societal expectations of the role of forests and forest ecosystem services. A survey using a digital questionnaire was conducted several months after the pandemic outbreak on a representative sample of the Slovak population. The Wilcoxon test and ordinal regression analysis were used to identify significant relationships, e.g., between the recency of anger episodes and the number of forest visits. The results showed that the pandemic strengthened the perception of forests as a high-quality restorative environment and that emotions associated with forest visits played an important role in the perceived importance of forests and their possible overexploitation. The results underscore the urgent need to put demands for forest recreation on par with the forest bioeconomy and to sensitize forest visitors to management and conservation requirements.

Spatiotemporal non-stationarity in green space and stress relationships: Recent evidence from South Korea.

A growing body of literature has linked exposure to "green space" (vegetation-rich areas) and other forms of nature to mental health. Exposure-outcome associations at regional or national scales can overlook local associations that define how geographically distinct populations may experience nature differently. Large-scale results might downplay the importance of lived experiences and heterogeneity of human-nature relationships at local scales. The current study examines three types of vegetative cover and identifies how they are associated with perceived stress in South Korea during and before the COVID-19 pandemic. We find forest cover is consistently negatively associated with perceived stress at nationwide scales. In contrast, grass cover and the normalized difference vegetation index (NDVI) show mixed associations with perceived stress at nationwide scales. Models accounting for spatial and temporal variability demonstrate that associations of forest cover, grass cover, and NDVI with perceived stress varies across the country and the study's four-year time horizon. Local governments may need divergent urban greening strategies for health promotion that respond to their specific sociodemographic and pre-existing environmental conditions.

COVID-19 contagion across remote communities in tropical forests.

Understanding COVID-19 contagion among poor populations is hampered by a paucity of data, and especially so in remote rural communities with limited access to transportation, communication, and health services. We report on the first study on COVID-19 contagion across rural communities without road access. We conducted telephone surveys with over 400 riverine communities in the Peruvian Amazon in the early phase of the pandemic. During the first wave (April-June, 2020), COVID-19 spread from cities to most communities through public and private river transportation according to their remoteness. The initial spread was delayed by transportation restrictions but at the same time was driven in unintended ways by government social assistance. During the second wave (August, 2020), although people's self-protective behaviors (promoted through communication access) helped to suppress the contagion, people responded to transportation restrictions and social assistance in distinct ways, leading to greater contagion among Indigenous communities than mestizo communities. As such, the spatial contagion during the early phase of the pandemic in tropical forests was shaped by river transportation and social behaviors. These novel findings have important implications for research and policies on pandemics in rural areas.

The effects of forest therapy on public mental health and circular economy: A policy support model in Japan.

Background: Forest therapy has gained popularity in Japan and even other nations/regions due to its health benefits. In addition, forest therapy has contributed to the development of circular economy and industrial upgrading. Japanese successful practice can serve as a model for other countries in the Asia-Pacific region. To this end, the aim of this study was to determine whether forest therapy can improve the whole well-being of the participants and has a positive effect on the development of circular economy in the region. Methods: Both empirical and inductive research methods were used; empirical approach was conducted to perform comparative analysis of regional data that was retrieved from the research project of Japanese Forestry Agency in 2015. Specifically, the efficacy of forest therapy on physical (blood glucose, blood pressure, body weight) and mental (sleep quality e.g.,) health outcomes among 815 participants was investigated. Regional data are from the statistics of Iiyama City from 1990 to 2005. After the concept of forest therapy became popular in the late 1990s, this element had a great positive impact on the economic benefits of Ishiyama City and other major forest scenic areas. We summarize and analyze a series of policies made by relevant departments of the Japanese government in the years from 2019 to 2021 to promote forest therapy and related circular industry development. Results: Significant (pre-to-post participation) changes in physical measure was observed. Firstly, mean weight of those overweight participants decreased across three different time points (pre-test/enrollment = 79.7 kg, 3-month participation = 77.2, and 6-month participation = 76.8 kg), while overall mean weight of the participants decreased to 61, 60.5, and 60.4 kg, respectively. Secondly, Participant with normal weight showed a decrease on mean HbA1C (from 6.09 to 6.06) at Week 24, while overweight participants demonstrated a slight change 6.03-6.01 after 6 months the average HOMA-IR for overweight participants decreased from 3.5 to 2.5 at Week 24, while participants with normal weight demonstrated a decrease from 2.2 to 1.7 at Week 24. Forest Therapy has emerged in Japan since Mid-1990s and has attracted a large number of tourists all over the world due to its unique health benefits. Conclusion: Forest therapy in Japan has positive effects on whole well-being of Japanese residents and it has helped public mental health promotion and economic growth. Under the guidance and support of government policies, it can promote the development of circular economy and industrial transformation and set a model of Japanese forest therapy development for other countries in the Asia-Pacific region.

The Feasibility and Impact of Practising Online Forest Bathing to Improve Anxiety, Rumination, Social Connection and Long-COVID Symptoms: A Pilot Study.

BACKGROUND: Long-COVID affects over 144 million people globally. In the absence of treatments, there is a need to establish the efficacy of therapies that improve patient outcomes. Forest bathing has been demonstrated to improve physical and mental outcomes but there is no evidence in Long-COVID patients. Accordingly, this pilot study sought to determine the feasibility and effectiveness of online forest bathing in adults with Long-COVID. METHODS: Feasibility was assessed by monitoring retention rates and participant feedback. In a waitlist controlled, repeated measures design, 22 Long-COVID patients completed weekly online surveys during a four-week waitlist control period, before engaging in four weekly online forest bathing sessions, completing post-intervention surveys following each session. RESULTS: In terms of retention, 27% did not provide post-intervention data, reasons for non-adherence were: feeling too ill, having medical appointments, or having career responsibilities. Compared with the waitlist control period, there were statistically significant improvements in Anxiety (49% decrease), Rumination (48% decrease), Social Connection (78% increase), and Long-COVID symptoms (22% decrease). Written qualitative comments indicated that participants experienced feelings of calm and joy, felt more connected socially and with nature, and experienced a break from the pain and rumination surrounding their illness. CONCLUSIONS: Online Forest bathing resulted in significant improvements in well-being and symptom severity and could be considered an accessible and inexpensive adjunct therapy for Long-COVID patients. Where people have limited access to in-person nature, virtual nature may offer an alternative to improve health and well-being outcomes.

Effects of forest environment (Shinrin-yoku/Forest bathing) on health promotion and disease prevention -the Establishment of "Forest Medicine".

Humans have enjoyed forest environments for ages because of the quiet atmosphere, beautiful scenery, mild climate, pleasant aromas, and fresh, clean air. In Japan, since 2004, serial studies have been conducted to investigate the effects of forest environments (Forest bathing/Shinrin-yoku) on human health. My research team has established a new medical science called Forest Medicine. The Forest Medicine is a new interdisciplinary science, belonging to the categories of alternative medicine, environmental medicine and preventive medicine, which studies the effects of forest environments (Forest bathing/Shinrin-yoku) on human health. It has been reported that Forest bathing/Shinrin-yoku has the following beneficial effects on human health:1 Shinrin-yoku increases human natural killer (NK) activity, the number of NK cells, and the intracellular levels of anti-cancer proteins, suggesting a preventive effect on cancers. 2 Shinrin-yoku reduces blood pressure and heart rate showing preventive effect on hypertension and heart diseases. 3 Shinrin-yoku reduces stress hormones, such as urinary adrenaline and noradrenaline and salivary/serum cortisol contributing to stress management. 4 Shinrin-yoku increases the activity of parasympathetic nerves and reduces the activity of sympathetic nerves to stabilize the balance of autonomic nervous system. 5 Shinrin-yoku improve sleep. 6 Shinrin-yoku increases the levels of serum adiponectin and dehydroepiandrosterone sulfate. 7 In the Profile of Mood States (POMS) test, Shinrin-yoku reduces the scores for anxiety, depression, anger, fatigue, and confusion, and increases the score for vigor, showing preventive effects on depression. 8 Shinrin-yoku may apply to rehabilitation medicine 9 Shinrin-yoku in city parks also has benefits on human health. 10 Shinrin-yoku may have preventive effect on COVID-19 by boosting immune function and by reducing mental stress.Taken together, these findings suggest that Shinrin-yoku may have potential preventive effects on non-communicable diseases.

Spatial variations in vegetation fires and emissions in South and Southeast Asia during COVID-19 and pre-pandemic.

Vegetation fires are common in South/Southeast Asian (SA/SEA) countries. However, very few studies focused on vegetation fires and the changes during the COVID as compared to pre-pandemic. This study fills an information gap and reports total fire incidences, total burnt area, type of vegetation burnt, and total particulate matter emission variations in SA/SEA during COVID-2020 and pre-pandemic (2012-2019). Results from the short-term 2020-COVID versus 2019-non-COVID year showed a decline in fire counts varying from - 2.88 to 79.43% in S/SEA. The exceptions in South Asia include Afghanistan and Sri Lanka, with a 152% and 4.9% increase, and Cambodia and Myanmar in Southeast Asia, with an 11.1% and 8.5% increase in fire counts in the 2020-COVID year. The burnt area decline for 2020 compared to 2019 varied from - 0.8% to 92% for South/Southeast Asian countries, with most burning in agricultural landscapes than forests. Several patches in S/SEA showed a decrease in fires for the 2020 pandemic year compared to long term 2012-2020 pre-pandemic record, with Z scores greater or less than two denoting statistical significance. However, on a country scale, the results were not statistically significant in both S/SEA, with Z scores ranging from - 0.24 to - 1, although most countries experienced a decrease in fire counts. The associated mean TPM emissions declined from ~ 2.31 Tg (0.73stdev) during 2012-2019 to 2.0 (0.65stdev)Tg in 2020 in South Asia and 6.83 (0.70stdev)Tg during 2012-2019 to 5.71 (0.69 stdev)Tg in 2020 for South East Asian countries. The study highlights variations in fires and emissions useful for fire management and mitigation.

A Comparative Study of Asymptomatic Malaria in a Forest Rural and Depleted Forest Urban Setting during a Low Malaria Transmission and COVID-19 Pandemic Period.

The global malaria morbidity and mortality witnessed an increase from 2019 to 2020 partly due to disruptions in control programs' activities imposed by the COVID-19 pandemic. Therefore, there is still a significant burden of malaria in Cameroon which needs attention from all fronts to attain elimination goals. It is normally expected that a typical forest ecology that has undergone urbanization and subjected to high rates of ecological instabilities should also have a shift from characteristic perennial malaria transmission and a shift in the type of malaria endemicity plaguing such distorted forest ecology. In this observational comparative study, we randomly enrolled participants from rural and urban settings of a forest zone during a low malaria transmission period, which coincided with the onset of COVID-19 pandemic. An optimized structured questionnaire was employed, to collect socio-demographic data and associated risk factors. The CareStart™ Malaria HRP2 antigen test was performed on participants from both settings to determine the prevalence of community asymptomatic malaria. Of 307 participants, 188 (61.0%) were from the rural, while 119 (38.8%) from the urban community. The overall prevalence of asymptomatic malaria (27.0%) detected Plasmodium falciparum antigen in 83 participants. The urban community's prevalence was 4.2% (5 positives) while the rural community's was 41.5% (78 positives). In simple logistic regression models, rural forest community and farm around the house were statistically significant predictors of testing positive (coefficient 2.8, 95% CI 1.8-3.7, p value<0.001) and (coefficient 3.1, 95% CI 1.1-5.1, p value =0.003), respectively. In the multivariate model, the strongest predictor of testing positive was living in a rural community, with p < 0.001 and odds ratio of 10.9 (95% CI, 3.8-31.8). These results indicate that during a low transmission period, the prevalence of asymptomatic malaria differs between depleted urban and rural forested settings, suggesting a need for strategic target intervention for the control of asymptomatic malaria.

Verification of Psychophysiological Effects of Satoyama Activities on Older Adult Volunteers and Young People in Post-COVID-19 Society: A Case Study of Matsudo City, Japan.

Many scholars have focused on Satoyama, which is characterized by mountains or villages away from the urban spaces. Our objective is to verify its psychophysiological effects on people performing usual Satoyama activities in ignored, small urban green spaces to help people find ways to stay healthy in post-coronavirus disease 2019 (COVID-19) society. In this study, 12 older adult volunteers from the "Forest around the Mountains" Nonprofit Organization and 12 young people from the university were invited as study subjects. They were asked to observe nature for 10 min and work for 30 min in the small green space "Forest around the Mountains". The Profile of Mood States 2nd Edition (POMS) and the State-Trait Anxiety Inventor (STAI) were used as psychological scales to detect their psychological restoration and blood pressure before and after the Satoyama activity. Their heart rate during the activity was used as the physiological indicator. The study showed that, as Satoyama volunteers, the older adults group had significant restorative psychophysiological effects during this experiment compared to the younger group; their systolic and diastolic blood pressure dropped significantly after Satoyama activities, On the other hand, the young group have been in the normotensive range before or after Satoyama activities The psychological indicators such as Anger-hostility, Confusion-bewilderment, and Tension-anxiety were significantly lower in the younger group but were still significantly higher than the indicators of the older adults. In general, this study found that Satoyama activities benefited young and older participants, especially older adults with high blood pressure. Satoyama activities in small urban green spaces are thus necessary and worth promoting in the post-COVID-19 era.

A participatory epidemiological and One Health approach to explore the community's capacity to detect emerging zoonoses and surveillance network opportunities in the forest region of Guinea.

The Ebola virus disease epidemic that threatened West Africa between 2013 and 2016 was of unprecedented health magnitude. After this health crisis, studies highlighted the need to introduce community-based surveillance systems and to adopt a One Health approach. This study aimed to provide preparatory insights for the definition of a community-based surveillance system for emerging zoonoses such as viral hemorrhagic fevers in Guinea. The objective was to explore the disease detection capacity and the surveillance network opportunities at the community level in two pilot areas in the forest region of Guinea, where the epidemic emerged. Based on a participatory epidemiological and One Health approach, we conducted Focus Group Discussions with human, animal and ecosystem health actors. We used a range of participatory tools, included semi-structured interviews, ranking, scoring and flow diagram, to estimate the local knowledge and perception of diseases and clinical signs and to investigate the existing health information exchange network and its related strengths and weaknesses. The results showed that there is heterogeneity in knowledge of diseases and perception of the clinical signs among actors and that there are preferred and more effective health communication channels opportunities. This preparatory study suggests that it is necessary to adapt the case definitions and the health communication channels to the different actors who can play a role in a future community-based surveillance system and provides recommendations for future surveillance activities to be carried out in West Africa.

COVID-19 information and self-protective behaviors among rural communities in tropical forests.

BACKGROUND: Health risk communication plays a key role in promoting self-protective measures, which are critical in suppressing COVID-19 contagion. Relatively little is known about the communication channels used by rural poor populations to learn novel measures and their effectiveness in promoting self-protective behaviors. Behavioral change can be shaped by people's trust in government institutions which may be differentiated by social identity, including indigeneity. METHODS: During an early phase of the pandemic, we conducted two telephone surveys with over 460 communities - both Indigenous and mestizo - without road access and limited communication access in the Peruvian Amazon. This is the first report on the association of information sources about self-protective measures against COVID-19 with the adoption of self-protective behaviors in remote rural areas in developing countries. RESULTS: People mainly relied on mass media (radio, television, newspapers) and interpersonal sources (local authorities, health workers, neighbors/relatives) for information and adopted handwashing, mask-wearing, social distancing, and social restrictions to varying degrees. Overall, self-protective behaviors were largely positively and negatively associated with mass media and interpersonal sources, respectively, depending on the source-measure combination. Mistrust of the government seems to have shaped how Indigenous and mestizo peoples distinctively responded to interpersonal information sources and relied on mass media. CONCLUSIONS: Our findings call for improved media access to better manage pandemics in rural areas, especially among remote Indigenous communities.

Balancing the scales: Preliminary investigation of total energy expenditure and daily metabolizable energy intake in Matschie's tree kangaroo (Dendrolagus matschiei).

Matschie's tree kangaroo (Dendrolagus matschiei) is an endangered arboreal marsupial native to Papua New Guinea. Detailed field studies of its behavior and ecology are scarce due largely to its occupation of remote cloud forests and cryptic nature. Although this species has been in human care since the 1950s, much of its biology is still unknown. The current ex situ population is not sustainable due to health and reproductive problems, believed to stem largely from issues with diet and obesity. To better assess potential discrepancies between energy requirements and energy intake, we sought to 1) quantify total energy expenditure (TEE) of two zoo-housed Matschie's tree kangaroos (body mass = 9.0-9.7 kg) on a diet composed largely of leafy browse; 2) quantify food and macronutrient intake, apparent dry matter macronutrient digestibility, and metabolizable energy (ME) intake over a 14-month period; and 3) test for seasonal changes in ME intake due to seasonal differences in the varieties of leafy browse offered. Using the doubly labeled water method, we determined TEE for the female (288 kcal day -1) and male (411 kcal day -1). Resulting mean TEE was well below the expected value for marsupials and macropods (i.e., ~60% of the expected value based on body mass). The mean calculated ME intakes for the female and male were 307 kcal day-1 and 454 kcal day-1, respectively. There were significant seasonal differences in ME intakes, driven by reduced intake in the autumn. These results demonstrate that Matschie's tree kangaroos can be maintained at healthy body weights and conditions on fiber-rich and browse-heavy diets. Our findings contribute important insights into tree kangaroo energetics and physiology and can be applied to help reformulate the diet of Matschie's tree kangaroos at captive facilities to improve population health and sustainability.

Preliminary investigation and intervention of the suspected plague outbreak in Madunga, Babati District-Tanzania.

BACKGROUND: Rodents are known to be reservoirs of plague bacteria, Yesinia pestis in the sylvatic cycle. A preliminary investigation of the suspected plague outbreak was conducted in Madunga Ward, Babati District Council in Manyara Region December-2019-January 2020 Following reported two cases which were clinically suspected as showing plague disease symptoms. METHOD: The commensal and field rodents were live trapped using Sherman traps in Madunga Ward, where plague suspect cases were reported and, in the Nou-forest reserve areas at Madunga Ward, Babati District Council, to assess plague risk in the area. Fleas were collected inside the houses using light traps and on the rodents 'body after anaesthetizing the captured rodent to determine flea indices which are used to estimate the risk of plague transmission. Lung impression smears were made from sacrificed rodents to examine for possible bipolar stained Yersinia spp bacilli. RESULTS: A total of 86 rodents consisting of ten rodent species were captured and identified from the study sites. Nine forest rodent species were collected. Field/fallow rodent species were dominated by Mastomys natalensis. whereas domestic rodent species captured was Rattus rattus. Overall lung impression smear showed bipolar stain were 14 (16.28%) while House Flea Index (HFI) was 3.1 and Rodent Flea Index (RFI) was 1.8. CONCLUSION: The findings of this study have shown that, the presence of bipolar stained bacilli in lung impression smears of captured species of rodents indicates (not confirmed) possible circulation of Yesrsinia pests in rodents and the high flea indices in the area which included the most common flea species known to be plague vectors in Tanzania could have played transmission role in this suspected outbreak. The study recommends surveillance follow-up in the area and subject collected samples to the standard plague confirmatory diagnosis.

High genetic diversity of alphacoronaviruses in bat species (Mammalia: Chiroptera) from the Atlantic Forest in Brazil.

Bat coronaviruses (Bat-CoVs) represent around 35% of all virus genomes described in bats. Brazil has one of the highest mammal species diversity, with 181 species of bats described so far. However, few Bat-CoV surveillance programmes were carried out in the country. Thus, our aim was to jevaluate the Bat-CoV diversity in the Atlantic Forest, the second biome with the highest number of bat species in Brazil. We analysed 456 oral and rectal swabs and 22 tissue samples from Atlantic Forest bats, detecting Alphacoronavirus in 44 swab samples (9.6%) targeting the RdRp gene from seven different bat species, three of which have never been described as Bat-CoV hosts. Phylogenetic analysis of the amino acid (aa) sequences coding the RdRp gene grouped the sequences obtained in our study with Bat-CoV previously detected in identical or congeneric bat species, belonging to four subgenera, with high aa identity (over 90%). The RdRp gene was also detected in three tissue samples from Diphylla ecaudata and Sturnira lilium, and the partial S gene was successfully sequenced in five tissues and swab samples of D. ecaudata. The phylogenetic analysis based on the partial S gene obtained here grouped the sequence of D. ecaudata with CoV from Desmodus rotundus previously detected in Peru and Brazil, belonging to the Amalacovirus subgenus, with aa identity ranging from 73.6% to 88.8%. Our data reinforce the wide distribution of Coronaviruses in bats from Brazil and the novelty of three bats species as Bat-CoV hosts and the co-circulation of four Alphacoronavirus subgenera in Brazil.